Publication details

Immunomodulatory Potency of Microcystin, an Important Water-Polluting Cyanobacterial Toxin

Investor logo
Authors

ADAMOVSKÝ Ondřej MOOSOVÁ Zdena PEKAROVÁ Michaela BASU Amrita BABICA Pavel ŠVIHÁLKOVÁ ŠINDLEROVÁ Lenka KUBALA Lukáš BLÁHA Luděk

Year of publication 2015
Type Article in Periodical
Magazine / Source Environmental Science & Technology
MU Faculty or unit

Faculty of Science

Citation
web http://pubs.acs.org/doi/abs/10.1021/acs.est.5b02049
Doi http://dx.doi.org/10.1021/acs.est.5b02049
Keywords Immunomodulatory Potency; Microcystin; Cyanobacterial Toxin
Description Microcystins (MCs) are primarily hepatotoxins produced by cyanobacteria and are responsible for intoxication in humans and animals. There are many incidents of chronic exposure to MCs, which have been attributed to the inappropriate treatment of water supplies or contaminated food. Using RAW 264.7 macrophages, we showed the potency of microcystin-LR (MC-LR) to stimulate production of pro-inflammatory cytokines (tumor necrosis factor alpha and interleukin-6) as a consequence of fast nuclear factor kappa B and nitrogen-activated protein kinase activation. In contrast to other studies, the observed effects were not attributed to the intracellular inhibition of protein phosphatases 1/2A due to lack of specific transmembrane transporters for MCs. However, the MC-LR-induced activation of macrophages was effectively inhibited by a specific peptide that blocks signaling of receptors, which play a pivotal role in the innate immune responses. Taken together, we showed for the first time that MC-LR could interfere with macrophage receptors that are responsible for triggering the above-mentioned signaling pathways. These findings provide an interesting mechanistic explanation of some adverse health outcomes associated with toxic cyanobacteria and MCs.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info