Publication details

β-secretase enzyme assay by MALDI TOF MS

Investor logo
Investor logo
Title in English ß-secretase enzyme assay by MALDI TOF MS
Authors

MACHÁLKOVÁ Markéta SCHEJBAL Jan GLATZ Zdeněk PREISLER Jan

Year of publication 2017
Type Conference abstract
Citation
Description Matrix assisted laser desorption/ionization mass spectrometry (MALDI MS) based method for ß-secretase enzyme assay was optimized in order to enable direct quantification of enzyme activity of ß-secretase. For this purpose MALDI MS-specific requirements for sample characteristics were reflected in optimized incubation parameters of studied enzyme assay. This allowed us to present an analytical method capable of direct quantification a product of enzyme reaction in the sample with no need for sample pretreatment and high degree of precision.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info