Publication details

Průkaz bakteriálních agens z hemokultur metodou fluorescenční in situ hybridizace

Title in English Identification of bacteria from blood cultures by fluorescence in situ hybridization
Authors

ČERNOHORSKÁ Lenka DOLINKOVÁ Lucie

Year of publication 2017
Type Article in Periodical
Magazine / Source Epidemiologie, mikrobiologie, imunologie
MU Faculty or unit

Faculty of Medicine

Citation
Field Epidemiology, infectious diseases and clinical immunology
Keywords sepsis; blood cultures; Gram stain; fluorescence in situ hybridization
Description Altogether 71 samples of positive blood cultures were tested. Blood cultures were detected in BACTEC analysator, than inoculated on solid media and growing bacteria were biochemicaly identified. Conformity between both methods was not hundred per cent, sensitivity of HemoFISH was 90,1 %. Good results were observed in case of staphylococci and enterobacteria. FISH identification failed in three cases, because hybridization probe were not able to bind to bacterial rRNA.

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