Publication details

Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry

Investor logo
Authors

ASMARI Mufarreh MICHALCOVÁ Lenka ALHAZMI Hassan A. GLATZ Zdeněk EL DEEB Sami

Year of publication 2018
Type Article in Periodical
Magazine / Source Microchemical Journal
MU Faculty or unit

Faculty of Science

Citation
Web http://dx.doi.org/10.1016/j.microc.2017.10.004
Doi http://dx.doi.org/10.1016/j.microc.2017.10.004
Field Biochemistry
Keywords Microscale thermophoresis; MS; biometals; deferiprone; CP20
Attached files
Description In this study, the microscale thermophoresis (MST) method was applied to investigate the interaction of deferiprone with biometals (Fe3+, Cu2+, Zn2+, Co2+, Ni2+, Mn2+, Mg2+, and Ca2+). The MST results indicated significant interactions of deferiprone with Fe3+, Cu2+, Zn2+, Co2+, and Ni2+. The data fitted to the Hill model shows stoichiometry more than 1:1. No binding was observed for deferiprone with Mn2+, Mg2+, and Ca2+. Electrospray ionization mass spectrometry confirms the results obtained by MST indicating stable molecular ion peaks for the complexes of deferiprone with either Fe3+, Zn2+, Cu2+, Co2+, Ni2+, and Mn2+, but no binding was observed for deferiprone with either Ca2+ and Mg2+ under a gas phase state. MST shows a fast and simple approach to study the binding of deferiprone to different essential metal ions. Moreover, the complexes were stable for investigation by mass spectrometry under a soft ionization technique like used electrospray ionization, which aided to confirm binding stoichiometry.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info