Publication details

Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide

Authors

SUN Xiaolong ODYNIEC Maria L. SEDGWICK Adam C. LACINA Karel XU Suying QIANG Taotao BULL Steven D. MARKEN Frank JAMES Tony D.

Year of publication 2017
Type Article in Periodical
Magazine / Source Organic Chemistry Frontiers
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://pubs.rsc.org/en/content/articlepdf/2017/qo/c6qo00448b
Doi http://dx.doi.org/10.1039/c6qo00448b
Keywords FLUORESCENT-PROBES; LIVING CELLS; BORONIC ACID; IN-VIVO; INFLAMMATION; REPORTER; MODEL
Description Based on the complexation of phenylboronic acid (PBA) with Alizarin Red S (ARS), we developed a new chemosensor for the detection of hydrogen peroxide (H2O2) in aqueous media. This platform has demonstrated its ability to detect H2O2 via colorimetric, fluorometric, and electrochemical measurements. The experimental observations reveal that the system displays a red-shifted visible colour change, on–off fluorescence response indicating release of indicator (ARS) and turn-on electrochemical signal indicating generation of phenol, after reaction with H2O2. With this work we have demonstrated that our reaction-based indicator displacement assay (RIA) systems, can be employed as an assay for H2O2 and hydrogen peroxide-related species for environmental and physiological detection.
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