Publication details
Lactobacillus caviae sp nov., an obligately heterofermentative bacterium isolated from the oral cavity of a guinea pig (Cavia aperea f. porcellus)
| Authors | |
|---|---|
| Year of publication | 2017 |
| Type | Article in Periodical |
| Magazine / Source | International Journal of Systematic and Evolutionary Microbiology |
| MU Faculty or unit | |
| Citation | |
| web | http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002044 |
| Doi | http://dx.doi.org/10.1099/ijsem.0.002044 |
| Keywords | Lactobacillus sp. nov.; oral cavity; guinea pig; MLSA |
| Description | A Gram-stain-positive, facultatively anaerobic, and catalase-and oxidase-negative bacterial strain designated MOZM2(T), having 98.4% 16S rRNA gene sequence identity with Lactobacillus reuteri DSM 20016(T), was isolated from a swab of the oral cavity of a home-bred guinea pig. Comparative analyses based on the hsp60, pheS and tuf genes confirmed L. reuteri as its closest relative species, with calculated sequence similarities of 92.8, 88.8 and 96.9 %, respectively. DNA-DNA hybridisation revealed a 42% degree of genetic similarity between the novel strain and L. reuteri DSM 20016(T). Strain MOZM2(T) degrades carbohydrates via the 6-phosphogluconate/phosphoketolase pathway, evidenced by its production of gas from glucose and the end products of hexose catabolism. Comparative analysis of the cellular fatty acid profiles determined significant differences between MOZM2(T) and L. reuteri DSM 20016(T) in their proportions of C-8:0, C-14:1, C-17:0, C-18:2 omega 6t and C-20:0 fatty acids. Results of genotypic analyses also demonstrated differences between these two strains. They also differed in DNA G +C content, and some biochemical and physiological characteristics. We therefore believe that the examined bacterial isolate should be considered as a new taxon within the group of obligately heterofermentative lactobacilli. The species name Lactobacillus caviae sp. nov. is proposed, of which the type strain is MOZM2(T) (= CCM 8609(T) = DSM 100239(T) = LMG 28780(T)). |