Publication details
Detekce protilátek proti Borrelia burgdorferi s.l. u volně žijících drobných savců a porovnání citlivosti přímých metod (PCR a kultivace)
| Title in English | Detection of antibodies against Borrelia burgdorferi s.l. in wild-type small mammals and comparison of susceptibility of direct methods (PCR and culture) |
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| Authors | |
| Year of publication | 2018 |
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| Citation | |
| Description | 775/5000 The aim of this study was to determine the prevalence of antibodies against Borrelia burgdorferi s.l. in wild-type small mammals as well as to compare the sensitivity of direct detection methods (cultivation and PCR). Between 2010 and 2014, 691 wild small mammals were seized in three locations in the Czech Republic. Serum (n = 351) or cardiac rinse (n = 340) were examined with a modified indirect enzyme immunoassay (ELISA). The seroprevalence did not differ (p higher than 0,05) according to the families (Muridae 10%, Microtidae 19%), sex (males 10%, females 14%) and localities (Poodří 11%, Moravský Kras 12% and Mohelno 14%). Based on the results, it is evident that B. burgdorferi s.l. circulating in the population of wild tiny in the given localities. The PCR method proved to be more sensitive compared to cultivation. |