Publication details
Heterochromatinization associated with cell differentiation as a model to study DNA double strandbreak induction and repair in the context of higher-order chromatin structure
| Authors | |
|---|---|
| Year of publication | 2014 |
| Type | Article in Periodical |
| Magazine / Source | Applied Radiation and Isotopes |
| Citation | |
| Doi | https://doi.org/10.1016/j.apradiso.2013.01.029 |
| Description | Cell differentiation is associated with extensive gene silencing, heterochromatinization and potentially decreasing need for repairing DNA double-strand breaks (DSBs). Differentiation stages of blood cells thus represent an excellent model to study DSB induction, repair and misrepair in the context of changing higher-order chromatin structure. We show that immature granulocytes form ?H2AX and 53BP1 foci, contrary to the mature cells; however, these foci colocalize only rarely and DSB repair is inefficient. Moreover, specific chromatin structure of granulocytes probably influences DSB induction. |