Publication details

UTILIZATION OF MICROPREPARATIVE FAST FOCUSING BY A NEW WIDE pH RANGE ELECTROLYTE SYSTEM BASED ON BIDIRECTIONAL ISOTACHOFORESIS

Authors

TESAŘOVÁ Marie DUŠA Filip HORKA Marie SLAIS Karel

Year of publication 2013
Type Article in Periodical
Magazine / Source CHEMICKÉ LISTY
MU Faculty or unit

Faculty of Science

Citation Chyba/Error
web http://www.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/3394/3350
Keywords electrolyte system
Description We suggest the possibility of practical utilization of a new electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis. The focusing occurs on nonwoven fabric strip positioned in an open horizontal V-shaped trough. It is based on bidirectional ITP with multiple counter ions and spacers created from commercially available simple buffers. Milk spiked with ampicillin was used as a sample and high performance liquid chromatography was as a second dimension for analysis of fast preparative focusing fractions. Speed, easy fraction handling, and possibility of pre-concentration of analytes from a raw sample are the benefits of this technique.

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