Publication details

The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis are Regulated through Oocyte Maturational DevelopmentA Complete Transcriptomic and Histochemical Study

Authors	CHERMULA Blazej BRAZERT Maciej JEŠETA Michal OZEGOWSKA Katarzyna SUJKA-KORDOWSKA Patrycja KONWERSKA Aneta BRYJA Artur KRANC Wieslawa JANKOWSKI Maurycy NAWROCKI Mariusz J. KOCHEROVA Ievgeniia CELICHOWSKI Piotr BOROWIEC Blanka POPIS Malgorzata BUDNA-TUKAN Joanna ANTOSIK Pawel BUKOWSKA Dorota BRUSSOW Klaus P. PAWELCZYK Leszek BRUSKA Malgorzata ZABEL Maciej NOWICKI Michal KEMPISTY Bartosz
Year of publication	2019
Type	Article in Periodical
Magazine / Source	International Journal of Molecular Sciences
MU Faculty or unit	Faculty of Medicine
Citation
web	http://dx.doi.org/10.3390/ijms20010084
Doi	http://dx.doi.org/10.3390/ijms20010084
Keywords	pig; oocytes; microarray; cellular competence
Description	The growth and development of oocyte affect the functional activities of the surrounding somatic cells. These cells are regulated by various types of hormones, proteins, metabolites, and regulatory molecules through gap communication, ultimately leading to the development and maturation of oocytes. The close association between somatic cells and oocytes, which together form the cumulus-oocyte complexes (COCs), and their bi-directional communication are crucial for the acquisition of developmental competences by the oocyte. In this study, oocytes were extracted from the ovaries obtained from crossbred landrace gilts and subjected to in vitro maturation. RNA isolated from those oocytes was used for the subsequent microarray analysis. The data obtained shows, for the first time, variable levels of gene expression (fold changes higher than \|2\| and adjusted p-value < 0.05) belonging to four ontological groups: regulation of cell proliferation (GO:0042127), regulation of cell migration (GO:0030334), and regulation of programmed cell death (GO:0043067) that can be used together as proliferation, migration or apoptosis markers. We have identified several genes of porcine oocytes (ID2, VEGFA, BTG2, ESR1, CCND2, EDNRA, ANGPTL4, TGFBR3, GJA1, LAMA2, KIT, TPM1, VCP, GRID2, MEF2C, RPS3A, PLD1, BTG3, CD47, MITF), whose expression after in vitro maturation (IVM) is downregulated with different degrees. Our results may be helpful in further elucidating the molecular basis and functional significance of a number of gene markers associated with the processes of migration, proliferation and angiogenesis occurring in COCs.