Generation of human iPSCs from fetal prostate fibroblasts HPrF

• Authors: KAHOUNOVA Zuzana; SLABAKOVA Eva; BINO Lucia; REMSIK Jan; FEDR Radek; BOUCHAL Jan; VRTEL Radek; JUREČKOVÁ Lucie; POROKH Volodymyr; PARALOVA Darja; HAMPL Aleš; SOUCEK Karel
• Year of publication: 2019
• Type: Article in Periodical
• Magazine / Source: Stem cell research
• MU Faculty or unit: Faculty of Medicine
• web: https://www.sciencedirect.com/science/article/pii/S1873506119300352
• Doi: http://dx.doi.org/10.1016/j.scr.2019.101405
• Keywords: iPSCs; fetal prostate fibroblasts
• Description: Human induced pluripotent stem cell line was generated from commercially available primary human prostate fibroblasts HPrF derived from a fetus, aged 18-24 weeks of gestation. The fibroblast cell line was reprogrammed with Yamanaka factors (OCT4, SOX2, c-MYC, KLF4) using CytoTune (TM)-iPS 2.0 Sendai Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting the expression of factors of pluripotency on a single-cell level, and in vivo using teratoma formation assay. This iPS cell line will be a useful tool for studying both normal prostate development and prostate cancer disease.

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