Publication details
Reduction of the DNA at a Pyrolytic Graphite Electrode:Opening of the Second Half of the Potential Window
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| Year of publication | 2018 |
| Type | Article in Proceedings |
| Conference | PROCEEDINGS OF THE INTERNATIONAL CONFERENCE MODERN ELECTROCHEMICAL METHODS XXXVIII |
| MU Faculty or unit | |
| Citation | |
| Keywords | 5-Methyleylosine; DNA; Electrochemistry; Electrochemical oxidation; Electrochemical reduction; Pyrolyticgraphite electrode; Uracil |
| Description | The pyrolytic graphite electrode (PGE) can be used at highly negative potentials, providing reduction signals known from mercury-based electrodes. Use of the negative part of the potential window more than doubled the number of analytically useful signals provided by unlabelled DNA on PGE. It enables selective analysis of all canonical DNA bases,5-methylcytosine and uracil It also allows for distinction of overlapping signals cathodic polarization can selectively "switch off" DNA anodic signals and rice versa. Newly observed signals corresponding to oxidation of reduced bases and reduction of oxidized bases are also described. |