Publication details

Enhanced Keratinocyte Cell Attachment to Atelocollagen Thin Films Through Air and Nitrogen Plasma Treatment

Authors

GARCIA Jorge Lopez PACHERNÍK Jiří LEHOCKY Marian JUNKAR Ita HUMPOLICEK Petr SAHA Petr

Year of publication 2011
Type Article in Proceedings
Conference TRENDS IN COLLOID AND INTERFACE SCIENCE XXIV
MU Faculty or unit

Faculty of Science

Citation
web https://link.springer.com/chapter/10.1007%2F978-3-642-19038-4_16
Doi http://dx.doi.org/10.1007/978-3-642-19038-4_16
Keywords Atelocollagen; Thin films; Plasma treatment; Cell adhesion; Cell growth; Radio frequency discharge
Description Collagen films (Atelocollagen from bovine Achilles tendon) were prepared in tissue culture dishes and their surfaces were modified by using air and nitrogen plasma treatment. The treated samples were characterised by surface probe techniques including attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy imaging (SEM) and X-ray photoelectron spectroscopy (XPS). In addition, human immortalised non-tumorigenic keratinocyte cell line (HaCaT) was seeded on the treated and untreated films and cell proliferation was measured by using MTT assay test. The characterisation results confirmed physical and chemical changes on the collagen surface, such as increase in the extent of surface oxidation and surface roughness as well as, the treated samples showed better cell growth than untreated ones, and therefore this approach may be taken into account in the development of promising materials for tissue regeneration applications.

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