Publication details
EFFECTS OF FLUOXETINE ON THYROID HORMONES METABOLISM
| Authors | |
|---|---|
| Year of publication | 2015 |
| Type | Article in Proceedings |
| Conference | RAD 2015: THE THIRD INTERNATIONAL CONFERENCE ON RADIATION AND APPLICATIONS IN VARIOUS FIELDS OF RESEARCH |
| MU Faculty or unit | |
| Citation | |
| Web | https://www.rad-conference.org/pdf/Proceedings%20RAD%202015.pdf |
| Keywords | antidepressant fluoxetine; radiometric enzyme assays; thyroid hormones |
| Description | In studies of the interaction of an antidepressant drug fluoxetine (Fluox) with the metabolism of thyroid hormones (TH) in the rat, we applied our newly developed radiometric assays for iodothyronine deiodinases (IDs) of types 1, 2 and 3 (D1, D2 and D3, respectively), using [125I]-labeled iodothyronines of high specific radioactivity as substrates. We followed in details the effects of administration during 25 days to adult Wistar rats of Fluox alone or in combination with triiodothyronine (T-3) on the production and degradation of TH in the CNS and in different peripheral rat tissues. Surprisingly, administration of Fluox in combination with supraphysiological amounts of T-3 caused a distinctive fall of non-physiologically high concentrations of serum total T-3, which were reached by giving the animals the same amounts of T-3 alone, to nearly normal levels. Profound changes in IDs enzyme activities were determined, especially in the pituitary and cerebellum of treated rats. In conclusion, the elaborated radiometric assays for IDs were found to be usable for the assessment of changes in these enzyme activities, caused by the administration of the studied drugs to the rats. |