Publication details

Transcriptomic analysis of expression of genes regulating cell cycle progression in porcine ovarian granulosa cells during short-term in vitro primary culture

Authors	KULUS M. KRANC W. SUJKA-KORDOWSKA P. CELICHOWSKI P. KONWERSKA A. JANKOWSKI M. JEŠETA Michal SKOWRONSKI M. T. PIOTROWSKA-KEMPISTY H. BUKOWSKA D. ZABEL M. BRUSKA M. MOZDZIAK P. KEMPISTY B. ANTOSIK P.
Year of publication	2020
Type	Article in Periodical
Magazine / Source	Histochemistry and Cell Biology
MU Faculty or unit	Faculty of Medicine
Citation
web	https://link.springer.com/content/pdf/10.1007/s00418-020-01860-2.pdf
Doi	http://dx.doi.org/10.1007/s00418-020-01860-2
Keywords	Pig; Ovarian follicle; Granulosa cells; Primary culture; Microarray
Description	The primary function of ovarian granulosa cells (GCs) is the support of oocytes during maturation and development. Molecular analyses of granulosa cell-associated processes, leading to improvement of understanding of the cell cycle events during the formation of ovarian follicles (folliculogenesis), may be key to improve the in vitro fertilization procedures. Primary in vitro culture of porcine GCs was employed to examine the changes in the transcriptomic profile of genes belonging to "cell cycle", "cell division", "cell cycle process", "cell cycle phase transition", "cell cycle G1/S phase transition", "cell cycle G2/M phase transition" and "cell cycle checkpoint" ontology groups. During the analysis, microarrays were employed to study the transcriptome of GCs, analyzing the total RNA of cells from specific periods of in vitro cultures. This research was based on material obtained from 40 landrace gilts of similar weight, age and the same living conditions. RNA was isolated at specific timeframes: before the culture was established (0 h) and after 48 h, 96 h and 144 h in vitro. Out of 133 differentially expressed genes, we chose the 10 most up-regulated (SFRP2,PDPN,PDE3A,FGFR2,PLK2,THBS1,ETS1,LIF,ANXA1,TGFB1) and the 10 most downregulated (IGF1,NCAPD2,CABLES1,H1FOO,NEK2,PPAT,TXNIP,NUP210,RGS2andCCNE2). Some of these genes known to play key roles in the regulation of correct cell cycle passage (up-regulated SFRP2, PDE3A, PLK2, LIF and down-regulated CCNE2, TXNIP, NEK2). The data obtained provide a potential reference for studies on the process of mammalian folliculogenesis, as well as suggests possible new genetic markers for cell cycle progress in in vitro cultured porcine granulosa cells.