Publication details

Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica

Authors

PAZOUREK Jiří ŠMEJKAL Karel

Year of publication 2016
Type Article in Periodical
Magazine / Source Molecules
MU Faculty or unit

Faculty of Pharmacy

Citation
Web https://www.mdpi.com/1420-3049/21/11/1495
Doi http://dx.doi.org/10.3390/molecules21111495
Keywords fraction collection; evaporative light scattering detector; hydrophilic interaction liquid chromatography; mixed-mode chromatography; Pectinatella magnifica
Description The use of a liquid chromatography (LC) splitter inserted between an HPLC column and an evaporative light scattering detector (ELSD) is described. This paper aims to show the feasibility of using the splitter in an HPLC-ELSD system to fractionate a model mixture of analytes, namely salicin (2-(hydroxymethyl)-phenyl-beta-D-glucopyranoside) and glucose. The retention factors and efficiency of the separation were studied under various temperatures and water contents in the mobile phase in order to clarify the mechanism of polyols separation on a diol column under the conditions of hydrophilic liquid chromatography (HILIC). Finally, the system was applied to a biological sample (a lyophilized colony gel of Pectinatella magnifica), where the presence of fructose and glucose was confirmed.

You are running an old browser version. We recommend updating your browser to its latest version.

More info