Publication details

Rapid HPLC method for monitoring of lactulose production with a high yield

Authors

PAZOUREK Jiří

Year of publication 2019
Type Article in Periodical
Magazine / Source Carbohydrate Research
MU Faculty or unit

Faculty of Pharmacy

Citation
Web https://www.sciencedirect.com/science/article/abs/pii/S0008621519302502
Doi http://dx.doi.org/10.1016/j.carres.2019.107773
Keywords Lactose; Lactulose production; Monitoring; Hydrophilic interaction liquid chromatography; Determination
Description An HPLC method suitable for rapid monitoring of lactulose production by isomerization from lactose was developed. The separation of lactose and lactulose under hydrophilic interaction liquid chromatography (HILIC) mode was achieved with resolution 1.5 within 5 min. Since isocratic elution was used, there is no extra time necessary for the column equilibration. Application of the method was illustrated on monitoring lactulose isomerization with catalysis of sodium hydroxide in the presence of sodium tetraborate at 70 °C (pH=11). The conversion yield obtained for lactulose was 86%, and corresponding purity 76%. For the first time, a polyhydroxy stationary phase for separation of lactose and lactulose is reported.

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