Publication details
Study of synthetic oligonucleotides containing guanine by circular dichroism spectroscopy and voltammetry on a pyrolytic graphite electrode
| Authors | |
|---|---|
| Year of publication | 2019 |
| Type | Article in Proceedings |
| Conference | PROCEEDINGS OF THE 15TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY |
| MU Faculty or unit | |
| Citation | |
| Keywords | circular dichroism spectroscopy; guanine; oxidation; voltammetry |
| Description | The possibility to study DNA quadruplex structures using voltammetric analysis of DNA hexamers was investigated using a pyrolytic graphite electrode in an acetate buffer (pH = 5.0). We have tested the influence of the hexamer sequence and DNA concentration their anodic oxidation. To verify the G quadruplex formation, or lack thereof, circular dichroism spectroscopy was used. Our results suggest that, on the pyrolytic graphite electrode, the guanine oxidation signals are not influenced by formation of G the quadruplex. Instead, the shape of the G oxidation multiple peak can be related to the primary structure of the oligonucleotide. |