Publication details
PREPARATION OF HYPERCROSSLINKED MONOLITHIC COLUMN FOR SEPARATION OF POLAR COMPOUNS
| Authors | |
|---|---|
| Year of publication | 2012 |
| Type | Article in Proceedings |
| Citation | |
| Keywords | Organic polymer monoliths; HILIC; Hypercrosslinking |
| Description | Hypercrosslinking, as a post-polymerization modification, allows preparation of high surface area organic polymer monoliths suitable for fast and efficient separation of small molecules in isocratic mode of capillary liquid chromatography. Infrared spectroscopy has confirmed that not all reactive chloromethyl groups in poly(styrene-co-chloromethyl styrene-co-divinylbenzene) monolithic capillary columns are consumed by hypercrosslinking modification. Remaining reactive chloromethyl groups allow following modification of monolithic surface. We have modified these remaining groups with thermally initiated 4,4'-azobis(4-cyanovaleric acid) initiator to produce activated hypercrosslinked surface. Once activated, almost any functional group can be attached to the hypercrosslinked monolith. We have used zwitterionic N,N-dimethyl-N-methacryloxyethyl-N-(3-sulfopropyl) ammonium betaine monomer to prepare a column allowing separation of polar compounds in hydrophilic interaction chromatography. The surface modification method has been optimized in terms of permeability, efficiency and selectivity of prepared monolithic capillary columns. The optimized column has been used for an isocratic and a gradient separation of proteins and phenolic acids. |