Publication details
In-cell NMR Spectroscopy of Nucleic Acids
| Authors | |
|---|---|
| Year of publication | 2020 |
| Type | Article in Periodical |
| Magazine / Source | IN-CELL NMR SPECTROSCOPY: FROM MOLECULAR SCIENCES TO CELL BIOLOGY |
| MU Faculty or unit | |
| Citation | |
| web | https://pubs.rsc.org/en/content/chapter/bk9781788012171-00272/978-1-78801-217-1 |
| Doi | http://dx.doi.org/10.1039/9781788013079 |
| Keywords | DNA; guanine quadruplex; nucleic acid; water |
| Description | The structure, dynamics, and interactions of nucleic acids, in particular DNAs, sensitively respond to a broad range of environmental factors, many of them being natively present in the intracellular space. In-cell NMR is a tailored adaptation of conventional high-resolution nuclear magnetic resonance spectroscopy that permits characterization of stereochemical features of nucleic acids directly in living cells. Here we review recent advances and applications of in-cell NMR to nucleic acids. The limitations of this method as well as the general considerations, comprising of critical factors to be accounted for during the planning of in-cell NMR experiments, as well as during the interpretation of in-cell NMR data, are discussed. |
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