Publication details

CD4(+) T Follicular Helper Cells in Human Tonsils and Blood Are Clonally Convergent but Divergent from Non-Tfh CD4(+) Cells

Authors	BRENNA E. DAVYDOV Alexey Nikolayevich LADELL K. MCLAREN J.E. BONAIUTI P. METSGER Maria RAMSDEN J.D. GILBERT S.C. LAMBE T. PRICE D.A. CAMPION S.L. CHUDAKOV Dmitriy BORROW P. MCMICHAEL A.J.
Year of publication	2020
Type	Article in Periodical
Magazine / Source	Cell Reports
MU Faculty or unit	Central European Institute of Technology
Citation
Web	https://linkinghub.elsevier.com/retrieve/pii/S2211124719316663
Doi	http://dx.doi.org/10.1016/j.celrep.2019.12.016
Keywords	GERMINAL CENTER RESPONSES; CXC CHEMOKINE RECEPTOR-5; B-CELLS; ANTIBODY-RESPONSES; INFLUENZA-VIRUS; HUMAN NAIVE; MEMORY; ANTIGEN; DIFFERENTIATION; INFECTION
Description	T follicular helper (Tfh) cells are fundamental for B cell selection and antibody maturation in germinal centers. Circulating Tfh (cTfh) cells constitute a minor proportion of the CD4(+) T cells in peripheral blood, but their clonotypic relationship to Tfh populations resident in lymph nodes and the extent to which they differ from non-Tfh CD4(+) cells have been unclear. Using donor-matched blood and tonsil samples, we investigate T cell receptor (TCR) sharing between tonsillar Tfh cells and peripheral Tfh and non-Tfh cell populations. TCR transcript sequencing reveals considerable clonal overlap between peripheral and tonsillar Tfh cell subsets as well as a clear distinction between Tfh and non-Tfh cells. Furthermore, influenza-specific cTfh cell clones derived from blood can be found in the repertoire of tonsillar Tfh cells. Therefore, human blood samples can be used to gain insight into the specificity of Tfh responses occurring in lymphoid tissues, provided that cTfh subsets are studied.