Publication details
Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography
| Authors | |
|---|---|
| Year of publication | 2014 |
| Type | Article in Periodical |
| Magazine / Source | Carbohydrate Research |
| MU Faculty or unit | |
| Citation | |
| Web | https://www.sciencedirect.com/science/article/abs/pii/S0008621514001050 |
| Doi | http://dx.doi.org/10.1016/j.carres.2014.03.010 |
| Keywords | myo-inositol; HILIC; ELSD |
| Description | A fast liquid chromatography method for separation and determination of myo-inositol is reported. Determination of the biologically important isomer of inositols, myo-inositol, was optimized to avoid overlapping to possible interferents according to European Pharmacopoeia (glycerol, D-mannitol) and saccharose. The method in HILIC mode is extremely selective to other carbohydrates which allows to separate myo-inositol from allo-and D-chiro-inositol with resolution 12.3 and 5.2, resp. and this way it enables to separate myo-inostiol from contingent carbohydrates present in a sample matrix. Retention time of myo-inositol was 12 min at 10 degrees C, though higher temperatures (25 degrees C or 40 degrees C) or higher water content in the mobile phase could speed up the separation and determination to four minutes. LOD of the method was 9 mg/L at 10 degrees C, and 5 mg/L at 25 degrees C, resp. (C) 2014 Elsevier Ltd. All rights reserved. |