Publication details

Cryopreserved buffy-coat-derived platelets reconstituted in platelet additive solution: A safe and available product with sufficient haemostatic effectiveness

Authors

LEJDAROVÁ Hana PACASOVÁ Rita PACASOVA Rita TESAŘOVÁ Eva TESAROVA Lenka KOUTNÁ Irena KOUTNA Irena POLOKOVÁ Naděžda POLOKOVA Nadezda MICHLÍČKOVÁ Simona MICHLICKOVA Simona DOLEČEK Martin

Year of publication 2021
Type Article in Periodical
Magazine / Source TRANSFUSION AND APHERESIS SCIENCE
MU Faculty or unit

Faculty of Medicine

Citation
web https://www.sciencedirect.com/science/article/pii/S1473050221000689
Doi http://dx.doi.org/10.1016/j.transci.2021.103110
Keywords Cryopreserved platelets; Buffy-coat-derived platelets; Platelet freezing; Platelet microparticles
Attached files
Description Background: Platelets (PLTs) stored at 20-24 degrees C have a short shelf life of only 5 days, which can result in their restricted availability. PLT cryopreservation extends the shelf life to 2 years. Methods: We implemented a method of PLT freezing at -80 degrees C in 5-6% dimethyl sulfoxide. Buffy-coat-derived leucodepleted fresh PLTs blood group O (FP) were used for cryopreservation. Cryopreserved pooled leucodepleted PLTs (CPP) were thawed at 37 degrees C, reconstituted in PLT additive solution SSP + and compared to FP regarding PLT content, PLT concentration, pH, volume, PLT loss, anti-A/B antibody titre, total protein, plasma content, and PLT swirling. Clot properties were evaluated via rotational thromboelastometry. PLT microparticle number and surface receptor phenotype were assessed via flow cytometry. Results: CPP met the required quality parameters. The mean freeze-thaw PLT loss was 22.24 %. Anti-A/B antibody titre and plasma content were significantly lower in CPP. CPP were characterised by faster clot initiation and form stable PLT clots. The number of PLT microparticles increased 25 times in CPP and there were more particles positive for the activation marker CD62 P compared to FP. Conclusion: Thawing and reconstitution are easy and fast processes if platelet additive solution is used. Low anti-A/B antibody titre and plasma content make possible the use of CPP of blood group O reconstituted in SSP + as universal ABO products, including clinical situations where washed PLTs are required. Clot properties evaluated via rotational thromboelastometry demonstrated that CPP retain a significant part of their activity compare to FP and are haemostatically effective.

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