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Kombinace laserové desorpce za účasti substrátu s ICP MS pro enzymové eseje

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Title in English Combination of substrate assisted laser desorptionand ICP MS for enzymatic assays
Authors

SVOJANOVSKÝ Vilém STIBOREK Marek KRÁSENSKÝ Pavel KROUPA Jiří KANICKÝ Viktor PREISLER Jan

Year of publication 2021
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description Substrate Assisted Laser Desorption (SALD) coupled with mass ICP MS spectrometry is a suitable technique for elemental analysis of samples deposited on plastic surfaces. 1,2 Immunochemical assays are a versatile immunoassay method for quantitative determination of a range of biologically relevant molecules that can be binding of a suitable antibody. These assays use enzymatic amplification or sensitive fluorescence or luminescence detection primarily in sandwich immobilised on a suitable surface. Use of nanoparticles as tags and ICP MS as a sensitive detector is a possible alternative to existing detection schemes. The aim of this work is to test SALD for the detection of nanoparticle-labeled antibodies from a polystyrene microtiter plate commonly used in immunochemical assays. In addition to the 96-well plate format, the a planar plate compatible with conventional laser cells ablation.
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