Publication details

DNA cycle sequencing of a common restriction fragment of Staphylococcus aureus bacteriophages by capillary electrophoresis using replaceable linear polyacrylamide

Investor logo
Authors

KLEPÁRNÍK Karel BERKA J. FORET F. DOŠKAŘ Jiří KAILEROVÁ Jana ROSYPAL Stanislav BOČEK Petr

Year of publication 1998
Type Article in Periodical
Magazine / Source Electrophoresis
MU Faculty or unit

Faculty of Science

Citation
Web
Field Genetics and molecular biology
Keywords Capillary electrophoresis; Staphylococcus aureus bacteriophage; DNA cycle sequencing; Linear polyacrylamide
Description The nucleotide sequence of a part of a 4.9 kbp common restriction fragment isolated from Staphylococcus aureus bacteriophage (bacterial virus) 3A has been determined by capillary electrophoresis (CE). The fast separation of sequencing fragments in linear polyacrylamide solution at a temperature of 55C allowed the reading of more than 650 bases of sequence in 60 min. The single strand (ss)DNA fragments were prepared by cycle sequencing with fluo-rescently labeled dideoxy-terminators on the cloned bacteriophage DNA template. With respect to analysis speed, sequence read-length, low sample consumption and automation, CE offers a simple, labor-saving and inexpensive procedure for DNA sequencing. Operating the CE columns at elevated temperature proved to be a rapid procedure capable of extending sequence read-length. The resulting sequence of the common restriction fragment can be used for the preparation of specific primers and oligonucleotide hybridization probes for identification of Staphylococcus aureus bacteriophages and/or prophages belonging to the bacteriophage species 3A
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info