Publication details
Role of Arachidonic Acid Metabolites in Proliferation, Differentiation and Apoptosis on Human Leukaemic Cell Line HL-60 after TPA Administration
| Authors | |
|---|---|
| Year of publication | 1999 |
| Type | Article in Proceedings |
| Conference | Cells |
| MU Faculty or unit | |
| Citation | |
| Field | Physiology |
| Description | TPA is known activator of protein kinase C. This activation subsequently reduces proliferation and induces differentiation and apoptosis of human myeloid leukaemia cell line HL-60. We have studied possible role of arachidonic acid metabolites in these processes. The reduction of proliferation and the induction of differentiation were not influenced either by indomethacin or MK-886. While indomethacin did not affect the percentage of apoptotic cells, MK-886 combined with TPA significantly enhanced the amount of apoptotic cells in concentration in which MK-886 alone did not induce apoptosis. Assuming that the enhancement of apoptosis by MK-886 was caused by 5-LPO inhibition, blocking the 5-LPO activity could contribute to leukaemia treatment. |