Publication details

Increased plasma cortisol level as acute response to glochidia parasitism

Authors

REICHARD Martin DOUDA Karel BLAŽEK Radim JANOVSKÁ Anna

Year of publication 2023
Type Article in Periodical
Magazine / Source Environmental Biology of Fishes
MU Faculty or unit

Faculty of Science

Citation
web https://doi.org/10.1007/s10641-022-01379-6
Doi http://dx.doi.org/10.1007/s10641-022-01379-6
Keywords Cost of parasitism; Freshwater bivalve; Glochidiosis; Hydrocortisone; Plasma cortisol; Unionida
Description Acute stress response in the European bitterling, Rhodeus amarus, to glochidia parasitism by the invasive unionid mussel, Sinanodonta woodiana, was quantified by analysing cortisol plasma levels using liquid chromatography coupled to mass spectrometer. We tested a novel method which required as little as 4 mu L of plasma by increasing the volume using charcoal-treated plasma. Bitterling were observed to increase cortisol levels significantly in response to glochidia infestation. Overall, this technique allows the precise measurement of steroid hormone plasma concentrations in small fish.

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