Publication details
Synthesis and modification of magnetic nanoparticles for bioanalytical applications
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| Year of publication | 2023 |
| Type | Conference abstract |
| Citation | |
| Description | Magnetic nanoparticles are attractive in bioanalysis, because their physical separation by a magnet from the sample is simple and they can be used for analyte preconcentration, provided the analyte interacts with their structure. Firstly, the magnetic nanoparticles were prepared via coprecipitation of iron salts by addition of ammonia, followed by the addition of a stabilizing agent – sodium citrate. The product was typically a brownish colloid, which has been sonicated to achieve uniform particle size - approximately 100 nm. The effect of supernatant removal and magnetic separation time was studied. The magnetic material was further modified, to obtain magnetic nanocomposites. To prepare a gold-iron oxide nanocomposite, modified Turkevich gold nanoparticle synthesis method, as well as a method developed by Lo et al. were used. The nanocomposites were of an orange-reddish and reddish-pink color, respectively. The first type of synthesized nanocomposites did not have a gold plasmon peak on UV-Vis spectra, however, the latter did, at 556 nm. The behavior of the bond between the iron oxide and gold nanoparticles was further studied. The future application of this product is selective and fast magnetic separation and preconcentration of biological thiols from biological fluid samples. Iron oxide nanoparticles were modified by silver as well, to use this material in surface-enhanced Raman spectrometry (SERS). The reduction of silver onto the magnetic core was performed by adding sodium borohydride. The size of the prepared silver-iron oxide nanocomposite was approximately 170 nm. The application of the prepared material to SERS seems to be possible since 100nM malachite green as well as 50µM riboflavin were detected. |