Publication details
Intravitální detekce a semi-kvantifikace hematofágních hlístic Ashworthius sidemi a Haemonchus spp. založená na qPCR
| Title in English | Intravital detection and semi-quantification of hematophagous nematodes Ashworthius sidemi and Haemonchus spp. based on qPCR |
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| Authors | |
| Year of publication | 2024 |
| MU Faculty or unit | |
| Web | https://botzool.sci.muni.cz/files/Final_CM_qPCR_As_Hc.pdf |
| Attached files | |
| Description | The presented methodology represents a standardized intravital diagnostic procedure for simultaneously detecting two pathogenic representatives of GIN parasitizing farmed or free-living ruminants. Specifically, it concerns representatives of the genus Haemonchus and their phylogenetically related, invasive species Ashworthius sidemi. The methodology is primarily designed to capture genera- or species-specific nucleic acid regions of both mentioned representatives of the parasites in the feces samples of their hosts during the patent period of infection. This is the period when nematode eggs are present in the feces of infected animals, which are released into the external environment and thus significantly contribute to the further spread of the infection. The advantage is that DNA of other origins can also be used for analysis in addition to feces. With the help of appropriately chosen isolation protocols, DNA can be extracted and tested from different developmental stages of nematodes (eggs, larvae, adults), infected host tissues, or the environment (e.g., vegetation). The advantage of the described diagnostic procedure is its multiplex potential, which makes it possible to detect several different genera or species of parasites in one quick reaction. Thus, the methodology represents a suitable tool for regular and coordinated monitoring of the occurrence and spread of these veterinary-important parasitic nematodes and early detection of infection in infected ruminants. Based on the diagnostic data obtained using this methodology, its user will be able to assess the effectiveness of the measures taken in the past and will be able to set the necessary preventive measures or therapeutic interventions. |
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