Publication details

An LRR receptor kinase controls ABC transporter substrate preferences during plant growth-defense decisions

Authors	ARYAL Bibek XIA Jian HU Zehan STUMPE Michael TSERING Tashi LIU Jie HUYNH John FUKAO Yoichiro GLOECKNER Nina HUANG Hsin -Yao SANCHO-ANDRES Gloria PAKULA Konrad ZIEGLER Joerg GORZOLKA Karin ZWIEWKA Marta NODZYNSKI Tomasz HARTER Klaus SANCHEZ-RODRIGUEZ Clara JASINSKI Michal ROSAHL Sabine GEISLER Markus M
Year of publication	2023
Type	Article in Periodical
Magazine / Source	Current Biology
MU Faculty or unit	Central European Institute of Technology
Citation
Web	https://www.sciencedirect.com/science/article/pii/S0960982223004773?via%3Dihub
Doi	http://dx.doi.org/10.1016/j.cub.2023.04.029
Keywords	plasma membrane proteins; auxin transport; necrotrophic pathogens; arabidopsis thaliana; phosphoproteomics
Description	The exporter of the auxin precursor indole-3-butyric acid (IBA), ABCG36/PDR8/PEN3, from the model plant Arabidopsis has recently been proposed to also function in the transport of the phytoalexin camalexin. Based on these bonafide substrates, it has been suggested that ABCG36 functions at the interface between growth and defense. Here, we provide evidence that ABCG36 catalyzes the direct, ATP-dependent export of camalexin across the plasma membrane. We identify the leucine-rich repeat receptor kinase, QIAN SHOU KINASE1 (QSK1), as a functional kinase that physically interacts with and phosphorylates ABCG36. Phosphorylation of ABCG36 by QSK1 unilaterally represses IBA export, allowing camalexin export by ABCG36 conferring pathogen resis-tance. As a consequence, phospho-dead mutants of ABCG36, as well as qsk1 and abcg36 alleles, are hyper-sensitive to infection with the root pathogen Fusarium oxysporum, caused by elevated fungal progression. Our findings indicate a direct regulatory circuit between a receptor kinase and an ABC transporter that func-tions to control transporter substrate preference during plant growth and defense balance decisions.