Publication details
Validation of Langendorff Parameters on Reperfusion Injury while Testing the Relationship Between Coronary Flow and Arrhythmia Rate
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| Year of publication | 2023 |
| Type | Appeared in Conference without Proceedings |
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| Description | The Langendorff isolated heart system has been used for many years in the scope of various myocardial studies. In our research, we aimed to test the role of external factors on the induction of ventricular arrhythmias in ex vivo rat hearts. All procedures and animal experiments were performed in full compliance with the ARRIVE and the European Community Council Directive (2010/63/EU) for Protection of Vertebrate Animals Used for Experimental and other Scientific Purposes guidelines. In this study, a total number of 12 male Wistar albino rats were used and divided into two groups according to body weight. The animals were anaesthetised using 2.5% isoflurane, and the anaesthesia was deepened until the loss of pedal reflexes. Later on, hearts were immediately excised and inserted into the Langendorff apparatus. K-H solution was used for the perfusion, and the system was set to 38 oC with constant oxygenation (95 O2/5 CO2) and pH set to 7.4. In the protocol, 40 minutes of regional ischemia was applied to LAD, followed by 5 minutes of reperfusion. 0,025 mg/ml adrenaline infused with 2.5 ml/hr rate during the last 5 minutes of ischemia, and the infusion rate is increased to 5 ml/hr during the reperfusion period. Lastly, the coronary flow was measured during the stabilization (CF1) and reperfusion (CF2) period for further examination. Depending on our results, group 1 rats with ± 201 g body weight with an average of 0,93 g of heart weight had 13 ml/min. CF1 and 16 ml/min. CF2. Group 1 rats showed 66 % of induction of ventricular fibrillation. Rats with ± 586 g body weight with an average of 2,29 g of heart weight had 20 ml/min. CF1 and 8 ml/min. CF2. Group 2 rats showed 66 % of induction of polymorphic ventricular tachycardia with no occurrence of fibrillation. In both groups, the induction of ventricular arrhythmias was 100 %. We can conclude that the hearts of rats with a smaller body weight showed a higher coronary flow and, as a result, a greater incidence of polymorphic ventricular tachycardia was recorded. In contrast, the hearts of rats with a higher body weight had a lower coronary flow and, as a result, a higher incidence of ventricular fibrillation. |