Publication details
Organotypic 3D Cell Culture of the Embryonic Lacrimal Gland
| Authors | |
|---|---|
| Year of publication | 2024 |
| Type | Chapter of a book |
| MU Faculty or unit | |
| Citation | KUONY, Alison, Matea BREZAK, René-Marc MÈGE and Zuzana SUMBALOVÁ KOLEDOVÁ. Organotypic 3D Cell Culture of the Embryonic Lacrimal Gland. In Zuzana Sumbalova Koledova. 3D Cell Culture: Methods in Molecular Biology. 2nd ed. New York: Humana New York, 2024, p. 145-156, 11 pp. volume 2764. ISBN 978-1-0716-3673-2. Available from: https://dx.doi.org/10.1007/978-1-0716-3674-9_10. |
| Description | Ectodermal organ development, including lacrimal gland, is characterized by an interaction between an epithelium and a mesenchyme. Murine lacrimal gland is a good model to study non-stereotypical branching morphogenesis. In vitro cultures allow the study of morphogenesis events with easy access to high-resolution imaging. Particularly, embryonic lacrimal gland organotypic 3D cell cultures enable the follow-up of branching morphogenesis thanks to the analysis of territories organization by immunohistochemistry. In this chapter, we describe a method to culture primary epithelial fragments together with primary mesenchymal cells, isolated from embryonic day 17 lacrimal glands. |