Publication details
Nová duální dehalogenáza-luciferáza z hvězdice křehké Amphiura filiformis
| Title in English | A new dual dehalogenase-luciferase enzyme from the brittle star Amphiura filiformis |
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| Authors | |
| Year of publication | 2024 |
| Type | Outcomes put into operation (prototype, working sample) |
| MU Faculty or unit | |
| web | https://loschmidt.chemi.muni.cz/peg/ |
| Description | We identified a gene, dafA, which encodes a protein with dehalogenase activity and low luciferase activity in the brittle star (Amphiura filiformis) genome. The gene dafA corresponds to two previously identified gene models (AF37308.1 and AFI06958.1). DafA exhibits luciferase activity that was significantly higher than the negative control, but produced light four and five orders of magnitude lower than that of AfLuc and Renilla luciferase (RLuc), respectively. Simultaneously, DafA exhibits dehalogenase activity which is comparable to those of characterized haloalkane dehalogenases in bacteria. |
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