Publication details
Nová vysoce aktivní luciferáza z hvězdice křehké Amphiura filiformis
| Title in English | A new highly active luciferase from the brittle star Amphiura filiformis |
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| Authors | |
| Year of publication | 2024 |
| Type | Outcomes put into operation (prototype, working sample) |
| MU Faculty or unit | |
| web | https://loschmidt.chemi.muni.cz/peg/ |
| Description | We identified a gene, afLuc, which encodes a protein with functional luciferase activity but no dehalogenase activity in the brittle star (Amphiura filiformis) genome. The gene afLuc corresponds to two previously identified gene models (Gen313061 and AFI20122.1). Based on a luciferase assay, which measures light production upon addition of coelenterazine, AfLuc exhibited statistically significant luciferase activity, compared to a negative control of bovine serum albumin. |
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