Publication details
Isolation of a Unique Monoterpene Diperoxy Dimer From Ziziphora clinopodioides subsp. bungeana Together With Triterpenes With Antidiabetic Properties
| Authors | |
|---|---|
| Year of publication | 2025 |
| Type | Article in Periodical |
| Magazine / Source | Phytochemical Analysis |
| MU Faculty or unit | |
| Citation | |
| web | https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/pca.3505 |
| Doi | http://dx.doi.org/10.1002/pca.3505 |
| Keywords | antidiabetic; GLUT4; pomolic acid; triterpenes; <italic>Ziziphora</italic>; alpha-glucosidase |
| Description | IntroductionZiziphora clinopodioides subsp. bungeana (Juz.) Rech.f. is used in traditional medicine for various purposes. Previous phytochemical studies focused on phenolic compounds, but triterpenoids were almost overlooked.ObjectiveThe study focused on the isolation of compounds with dual antidiabetic activity from the aerial parts of Z. clinopodioides subsp. bungeana.Materials and MethodsSeparation of CHCl3-soluble fraction by silica gel column chromatography using different mobile phases and purification of compounds by semi-preparative HPLC or preparative TLC. The structures of pure compounds were elucidated by 1D and 2D NMR experiments along with HRMS. Compound 1 was additionally identified by the single crystal X-ray diffraction method. alpha-Glucosidase inhibitory assay and GLUT4 expression and translocation in C2C12 myotubes were conducted to evaluate antidiabetic potential of isolated compounds.ResultsThis phytochemical study led to the isolation of 20 compounds, including a unique monoterpene diperoxy dimer (1). Compounds 7 and 9-11 displayed more potent alpha-glucosidase inhibitory activity (IC50 45.3-135.3 mu M) than acarbose used as a positive control (IC50 264.7 mu M), while only pomolic acid (5) increased GLUT4 translocation in C2C12 myotubes in a significant manner.ConclusionExtensive chromatographic separation led to the isolation and identification of a unique monoterpene diperoxy dimer (1) from aerial parts of Z. clinopodioides subsp. bungeana. Some triterpenes inhibited alpha-glucosidase, another increased GLUT4 translocation. Although none of the isolated compounds demonstrated dual antidiabetic activity, selected triterpenes proved to be potent antidiabetic agents in vitro. |
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