Publication details
Amplification of nuclear markers of early medieval pig ancient DNA from south Moravia
| Authors | |
|---|---|
| Year of publication | 2024 |
| Type | Article in Periodical |
| Magazine / Source | Research in Pig Breeding |
| MU Faculty or unit | |
| Citation | |
| web | http://www.respigbreed.cz/2024/2/1.pdf |
| Keywords | pig aDNA; ancient DNA; Middle Ages; PCR |
| Description | Ancient DNA (aDNA) is a DNA molecule older than 75 years. This molecule is characterized by many special properties. The main characteristics include its fragmentation. The short fragment length is often a limitation of the analysis. In this study, we analyzed historical animal samples from the Pohansko site (South Moravia, Czech Republic), which is dated to the 10th century. Two pig tooth samples were selected for aDNA extraction. After successful isolation, three nuclear DNA markers were amplified by PCR. These markers were selected due to the length of their amplification products (134 bp, 209 bp, and 312 bp, respectively). The amplification of the markers was successful for the HAL gene in one sample, and for the TEAD3 gene in both samples, the CYP2E1 gene was not successfully amplified in any sample. |