Publication details
Seed and embryo imaging: what works and what doesn't
| Authors | |
|---|---|
| Year of publication | 2024 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Flowering plants produce seeds, one of the bases of our food. However, instances of climate change with increased temperatures and the occurrence of heat waves deeply affect seed production. In this lecture, I will present different imaging technologies to assess resilience to stress during seed development. I will catalogue the different imaging methodologies used in our laboratory to assess seed and embryo development in Arabidopsis thaliana, Brassica napus and tomato. Seeds are imaged for morphological assessment or assessment of biosensors. It requires specific equipment such as light, fluorescence, confocal microscopes, or X-ray computer tomography. Samples may have to be prepared (fixed and sectioned, cleared). Examples of what works and what does not work in our hands will be provided. |