Stanovení metallothioneinu v tkáních okouna říčního a kapra obecného(Perca fluviatilis L., Cyprinus carpio L.)

• Title in English: Detection of metallothionein in fish tissues-Perca fluviatilis L., Cyprinus carpio L.
• Authors: KNÁPEK Jan; KONEČNÁ Marie; JARKOVSKÝ Jiří; TRNKOVÁ Libuše; KIZEK René
• Year of publication: 2001
• Type: Article in Proceedings
• Conference: Sborník:5.pracovní setkání biochemiků a molekulárních biologů, Brno 2001
• MU Faculty or unit: Faculty of Science
• Field: Electrochemistry
• Keywords: Perca fluviatilis L.; Cyprinus carpio L.; metallothionein; Chronopotentiometric stripping analysis; catalytic process in hydrogen evolution
• Description: Metalloproteins are low molecular proteins with catalytic and homeostatic function. The aim of this work was the quantification of metallothionein (MT)which produces at HMDE a well developed catalytic signal(peak H). The highest sensitivity of the MT determination was obtained with CPSA. Due to catalytic hydrogen evolution at highly negative potentials, picomolar concentrations of MT in various tissues were found.

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