Publication details
Biophysical analysis of natural, double-helical DNA modified by anticancer heterocyclic complexes of ruthenium(III) in cell-free media
| Authors | |
|---|---|
| Year of publication | 2001 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Biological Inorganic Chemistry |
| MU Faculty or unit | |
| Citation | |
| Field | Biophysics |
| Keywords | DNA; ruthenium; cytostatika; interakce |
| Description | Modifications of natural DNA by three anticancer heterocyclic ruthenium(III) compounds were studied by methods of molecular biophysics. These methods include: DNA binding studies using atomic absorption spectrophotometry, inhibition of restriction endonucleases, mapping of DNA adducts by transcription assay, interstrand cross-linking employing gel electrophoresis under denaturing conditions, DNA unwinding studied by gel electrophoresis, circular dichroism analysis of B to Z transition in DNA and DNA melting curves measured by absorption spectrophotometry. It has been suggested that the altered DNA binding mode of ruthenium compounds in comparison with cisplatin might be an important factor responsible for altered cytostatic activity of this class of ruthenium compounds in tumor cells. |