Publication details

Evaluation of ribotyping for characterization and identification of <I>Enterococcus haemoperoxidus</I> and <I>Enterococcus moraviensis</I> strains

Authors

ŠVEC Pavel SEDLÁČEK Ivo PANTŮČEK Roman DEVRIESE Luc DOŠKAŘ Jiří

Year of publication 2001
Type Article in Periodical
Magazine / Source FEMS Microbiology Letters
MU Faculty or unit

Faculty of Science

Citation
web [Abstract]
Field Genetics and molecular biology
Keywords Identification; Ribotyping; Enterococcus haemoperoxidus; Enterococcus moraviensis
Description Seven Enterococcus moraviensis and 16 Enterococcus haemoperoxidus as well as nine reference cultures of other enterococcal species obtained from the Czech Collection of Microorganisms were characterized using ribotyping with EcoRI and HindIII in the present work. The ribopatterns obtained by both restriction enzymes clearly distinguished all E. moraviensis and E. haemoperoxidus strains from the other enterococci (E. faecalis, E. faecium, E. avium, E. raffinosus, E. pseudoavium, E. malodoratus) and they differentiated both species from each other as well. Although all strains were isolated from different sampling sites, many strains shared the same band patterns. E. moraviensis formed four ribogroups using EcoRI and two ribogroups using HindIII restriction enzyme. E. haemoperoxidus gave six different patterns with EcoRI and five using the HindIII restriction enzyme.
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