Publication details
Measurement of small scalar and resiudal dipolar couplings in purine and pyrimidine bases
| Authors | |
|---|---|
| Year of publication | 2001 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Biomolecular NMR |
| MU Faculty or unit | |
| Citation | |
| Field | Physical chemistry and theoretical chemistry |
| Keywords | NMR methods; nucleic acid bases; residual dipolar couplings; spin-state-selective filter |
| Description | A suite of spin-state-selective excitation (S3E) NMR experiments for the measurements of small one-bond (13C - 13C, 13C - 15N) and two-bond (1H - 13C, 1H - 15N) coupling constants in 13C, 15N labeled purine and pyrimidine bases is presented. The incorporation of band-selective shaped pulses, elimination of the cross talk between á and â sub-spectra, and accuracy and precision of the proposed approach are discussed. Merits of using S3 E rather than á/â-half-filter are demonstrated using results obtained on isotopicaly labeled DNA oligonucleotides. |
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