Properties of nucleases immobilized on hydrophilic polymer particles

• Authors: RITTICH Bohuslav; ŠPANOVÁ Alena; RUDOLF Ivo; OHLASHENNYY Yuri; HORÁK Daniel; LENFELD Jiří; BENEŠ Milan J.
• Year of publication: 2001
• Type: Article in Proceedings
• Conference: Separation in the BioSciences SBS 2001
• MU Faculty or unit: Faculty of Science
• Field: Biotechnology
• Keywords: magnetic cellulose; magnetic poly(HEMA-co-EDMA); immobilized DNaseI; bacterial and plasmid DNAs
• Description: DNaseI immobilized on two hydrophilic supports in the form of microspheres, magnetic cellulose and magnetic poly(HEMA-co-EDMA) was used.Activity of immobilized DNaseI was estimated spectrophotometrically and confirmed by agarose gel electrophores. Effet of DNaseI activated with different metal ions on hydrolysis of both chromosomal and plasmid DNA was studied.

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