Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles

• Authors: RITTICH Bohuslav; ŠPANOVÁ Alena; OHLASHENNYY Yuriy; RUDOLF Ivo; HORÁK Daniel; LENFELD Jiří; BENEŠ Milan J.
• Year of publication: 2001
• Type: Article in Proceedings
• Conference: The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists
• MU Faculty or unit: Faculty of Science
• Field: Microbiology, virology
• Keywords: immobilization; DNase I; activity; stability; applications
• Description: The aim of work has been concentrated on degradation of high-weight and plasmid DNAs using immobilized DNase I. The activity of free and immobilized DNase I was estimated from UV spectrophotometric measurements and from agarose gel electrophoresis. The immobilized enzyme was full actived using Co2+ ions as an activator, a decrease of the activity was observed using other ions. Practical applications of DNase I immobilized on poly(HEMA-co-EDMA) microspheres in cleavage of plasmid DNAs are discussed.

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