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Vnitrobuněčné inhibitory PCR v baktériích Salmonella typhimurium
Title in English | Intracellular PCR inhibitors in bacteria Salmonella typhimurium |
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Authors | |
Year of publication | 2001 |
Type | Article in Proceedings |
Conference | V. Pracovní setkání biochemiků a molekulárních biologů |
MU Faculty or unit | |
Citation | |
Field | Microbiology, virology |
Keywords | PCR; inhibitors; Salmonella typhimurium |
Description | The aim of the work was to determine sensitivity of PCR detection of bacterial cells Salmonella typhimurium LT2-18 and Salmonella typhimurium LB5000. DNA prepared using phenol extraction methods and DNA from boiled cell lysates was used in PCR. Remarkable differences in PCR sensitivity were found out in tested strains if DNA cell lysates was used. |
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