Publication details

Vnitrobuněčné inhibitory PCR v baktériích Salmonella typhimurium

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Title in English Intracellular PCR inhibitors in bacteria Salmonella typhimurium
Authors

PRODĚLALOVÁ Jana ŠPANOVÁ Alena

Year of publication 2001
Type Article in Proceedings
Conference V. Pracovní setkání biochemiků a molekulárních biologů
MU Faculty or unit

Faculty of Science

Citation
Field Microbiology, virology
Keywords PCR; inhibitors; Salmonella typhimurium
Description The aim of the work was to determine sensitivity of PCR detection of bacterial cells Salmonella typhimurium LT2-18 and Salmonella typhimurium LB5000. DNA prepared using phenol extraction methods and DNA from boiled cell lysates was used in PCR. Remarkable differences in PCR sensitivity were found out in tested strains if DNA cell lysates was used.
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