Publication details
Capillary Array Electrophoresis-MALDI Mass Spectrometry Using a Vacuum Deposition Interface
| Authors | |
|---|---|
| Year of publication | 2002 |
| Type | Article in Periodical |
| Magazine / Source | Analytical Chemistry |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | capillary array electrophoresis; MALDI; mass spectrometry; proteomics; peptide |
| Description | The approach of vacuum deposition has been extended to demonstrate parallel analysis for multiple on-line infusion MALDI TOF MS and capillary array electrophoresis (CAE)-MALDI MS. In the infusion mode, individual peptide samples were simultaneously deposited on a Mylar tape cartridge using an array of eight capillaries, yielding eight parallel traces. For CAE-MALDI/TOF MS, the same number of separation capillaries were coupled with an array of eight infusion capillaries using a common liquid junction, containing matrix solution. A fast-scanning mirror was employed to traverse the beam of the desorption laser across the Mylar tape to probe one trace at a time. The positions of the eight sample traces formed on the tape were automatically determined, and all samples were analyzed in rapid sequence using a kilohertz repetition rate laser and a high-throughput data acquisition system. The instrumentation was operated with CAE MS for high-throughput analysis without compromising data quality applicable to MALDI/TOF MS analysis for proteomics and other areas where separation and high throughput are required. |