Publication details
Stanovení azidothymidinu v lidské moči, krevním séru a krvi
| Title in English | Determination of azidothymidine in human urine, serum and whole blood |
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| Authors | |
| Year of publication | 2002 |
| Type | Article in Proceedings |
| Conference | Sborník z VI. pracovního setkání biochemiků a molekulárních biologů |
| MU Faculty or unit | |
| Citation | |
| Field | Electrochemistry |
| Keywords | AZT;Electrochemical methods;Electroanalysis;Reduction of azides;Detection limit of AZT |
| Description | Azidothymidine (AZT), antiviral drug, was studied in human body liquids(urea,serum and whole blood)by electrochemical methods in connection with hanging mercury electrode(HMDE). Reduction signals of AZT (~1.1 V) obtained using cyclic (CV), differential pulse (DPV, square wave(SWV)and chronopotentiometric stripping analysis (CPSA)were compared.The most suitable method for the AZT determination was SWV where the optimalization of experimental conditions was made. The detection limit AZT in phosphate buffer was 500 pM. It was found that the square wave voltammetry is very fast and chief method for quantification of AZT in body humour. It is able to study the metabolism of this drug and farmacological monitoring of AZT in the case of HIV positive pacients. |
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