Publication details

Use of molecular-biological methods and HPLC for discrimination of Armillaria species in Czech Republic

Authors

LOCHMAN Jan ŠERÝ Omar JANKOVSKÝ Libor MIKEŠ Vladimír

Year of publication 2002
Type Article in Proceedings
Conference Book of Abstracts of the congress EFPP
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords Armillaria; discrimination; ITS; PCR
Description More than 40 isolates containing six species of Armillaria from the collections have been analyzed (A. borealis, A. cepistipes, A. gallica, A. mellea, A. ostoyae and A. tabescens) using the PCR of the internal transcribed spacer (ITS). The amplificates were further analysed by RFLP (Alu I, Hinf I and Mbo I) and electrophoreticaly separated both on 3% agarose gel and by IEC- HPLC on TSKgel DEAE-NPR column (Supelco). Digestion with Hinf I allowed discrimination of all six species.
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