Publication details

Utilisation of nonenzymatic hydrolysis of DNAs for characterisation of extrachromosomal elements in different strains of Bifidobacteria

Investor logo
Authors

ŠPANOVÁ Alena RITTICH Bohuslav

Year of publication 2002
Type Article in Proceedings
Conference Seventh Symposium on Lactic Acid Bacteria
MU Faculty or unit

Faculty of Science

Citation
Field Microbiology, virology
Keywords Nonenzymatic hydrolysis; extrachromosomal elements; Bifidobacteria
Description The nonenzymatic DNA hydrolysis of plasmid DNA in the presence of Eu3+ and La3+ ions can lead to single- and double-stranded breaks in DNA. The cleaved plasmid products can be easily separated and characterised by agarose gel electrophoresis. The nonenzymatic DNA cleavage was used in characterisation of extrachromosomal elements of bacteria of the genus Bifidobacterium.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info