Publication details
Immunomagnetic separation of Salmonella cells using newly designed magnetic carriers
| Authors | |
|---|---|
| Year of publication | 2002 |
| Type | Article in Proceedings |
| Conference | 22nd International Symposium on the Separation of Proteins, Peptides and Polynucleotides |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | Poly(HEMA-co-EDMA) magnetic microsheres; immunomagnetic separation; Salmonella |
| Description | Salmonella cells can be detached using immonomagnetic particles from competitive microflora or environments containing extracellular PCR inhibitors. As comercially avaiable Dynabeads anti-Salmonella magnetic particles are characterised by hydrophobic properties, magnetic hydrophilic non-porous microspheres, 1.5um in size, were developed. They were prepared by dispersion copolymerisation of 2-hydroxyethylmethacrylate and ethylene dimethacrylate (poly(HEMA-co-EDMA))or glycidylmethacrylate (poly(HEMA-co-GMA)). Such particles were functionalised by specific Salmonella antibodies and successfully used in IMS-CM and IMS-PCR arrangements. |
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