Publication details
Studium nádorově supresivních účinků proteinu p53 na leukemické monoblasty BM2
| Title in English | The study of tumor suppressive effects of p53 protein on leukemic monoblasts BM2 |
|---|---|
| Authors | |
| Year of publication | 2002 |
| Type | Article in Proceedings |
| Conference | VI. Pracovní setkání biochemiků a molekulárních biologů |
| MU Faculty or unit | |
| Citation | |
| Field | Oncology and hematology |
| Keywords | p53; BM2; monoblast; roscovitine |
| Description | We created a transgenic variant of the BM2 cell line of v-myb-transformed monoblasts inducibly expressing human p53. Transcription activation function of p53 as well as its intracellular concentration is kept low under regular conditions. DNA damage or another stressing attack cause increase of p53 stability and activation of its capability to transactivate target genes. The level of p53 decreases in 24 hours to its original amount during 24 hours in BM2p53 cell in spite of continuous presence of inducer. The temporal increase of p53 level did not affect growth and/or apoptosis of BM2p53 cells. However, rosocovitine- or adriamycine-treatment increased stability of the p53 protein causing lower viability of BM2p53 cells. At the same time molecular weight of p53 increased. |
| Related projects: |